Application

ELISA (1:2000)r>Flow Cytometry (1:1000)r>Immunoblotting (1:1000)r>Immunofluorescence (1:200)r>Immunohistochemistry (1:250)r>Immunoprecipitation (1:200)r>Neutralization Studies (see comments and application references)r>Radioimmunoassay (1:5000)

General description

Rat monoclonal antibody purified by ammonium sulfate precipitation and DEAE chromatography. Recognizes the ~85-95 kDa CD44 protein.

Anti-CD44, rat monoclonal, clone A020, recognizes the ~85-95 kDa CD44 protein (gp90) in human and GP85 in mouse. It is validated for ELISA, IF, IP, RIA, WB, and IHC.

Recognizes the ~85-95 kDa CD44 protein (gp90) in human and GP85 or Pgp-1 in mouse.

Immunogen

Human

purified human lymphocyte CD44

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

The antibody specifically recognizes and reacts with a common determinant of the CD44 class of glycoproteins. This antibody has been reported to block the interaction between hyaluronan and CD44 (see application references). Variables associated with assay conditions will dictate the proper working dilution.

Iida, N., and Bourguignon, L.Y.W. 1995. J. Cell Physiol.162, 121.Bourguignon, L.Y.W., et al. 1993. J. Immunol.151, 6634.

Packaging

100 µL in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)